We will employ in situ labeling of natural oviposition sites with Rhodamine B in two defined areas of Miami-Dade County that are candidate sites for an intervention study. This approach circumvents the need to rear (from lab or field-derived egg collections), mark, and release mosquitoes and may be more acceptable to communities in Miami-Dade. Dr. Ryan will provide support in modeling spatio-temporal movement of aegypti in the release sites. By using natural breeding sites this mark-capture approach uses natural mosquito releases from these label-enriched oviposition sites, and therefore does not contribute additional mosquitoes to the environment.